| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
|
| Autores: |
Marti, R.
Mieszkin, Sophie
Solecki, O.
Pourcher, A. -m.
Hervio-heath, Dominique
Gourmelon, Michele
|
| Data: |
2011-11
|
| Ano: |
2011
|
| Palavras-chave: |
Lactobacillus amylovorus
Microbial source tracking
Oxygen
Persistence
Pig-associated Bacteroidales markers
Quantitative real-time PCR
River water
Temperature
|
| Resumo: |
Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00053/16470/14534.pdf
DOI:10.1111/j.1365-2672.2011.05131.x
|
| Editor: |
Wiley-blackwell
|
| Relação: |
http://archimer.ifremer.fr/doc/00053/16470/
|
| Formato: |
application/pdf
|
| Fonte: |
Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2011-11 , Vol. 111 , N. 5 , P. 1159-1175
|
| Direitos: |
2011 The Authors. Journal of Applied Microbiology 2011 The Society for Applied Microbiology
|
|
